F5875
Ferredoxin from Spinacia oleracea (spinach)
buffered aqueous solution
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About This Item
Recommended Products
biological source
spinach (Spinacia oleracea)
Quality Level
form
buffered aqueous solution
concentration
1.0-3.0 mg/mL in 0.15 M Trizma buffer, pH 7.5 and NaCl (Frozen)
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
spinach ... SOVF(137530)
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Application
Ferredoxin has been used in studies of assimilation of inorganic nitrogen from nitrite or nitrate in the absence of ammonium. Ferredoxin is important in the electron transfer cascade required for this nitrogen assimilation.
Biochem/physiol Actions
Ferredoxins are iron-containing proteins that serve as electron-transfer catalysts in photosynthesis and nitrogen fixation.
Packaging
Package sizes are based on ferredoxin content.
Preparation Note
Partially purified by the method of Tagawa and Arnon, Nature, 195, 537 (1962).
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Rosa M Martínez-Espinosa et al.
Biochimica et biophysica acta, 1623(1), 47-51 (2003-09-06)
Haloferax mediterranei is a halophilic archaeon that can grow in aerobic conditions with nitrate as sole nitrogen source. The electron donor in the aerobic nitrate reduction to ammonium was a ferredoxin. This ferredoxin has been purified and characterised. Air-oxidized H.
Basilio Zafrilla et al.
Biochemical Society transactions, 39(6), 1844-1848 (2011-11-23)
In the absence of ammonium, many organisms, including the halophilic archaeon Haloferax volcanii DS2 (DM3757), may assimilate inorganic nitrogen from nitrate or nitrite, using a ferredoxin-dependent assimilatory NO₃⁻/NO₂⁻ reductase pathway. The small acidic ferredoxin Hv-Fd plays an essential role in
Rosa María Martínez-Espinosa et al.
FEMS microbiology letters, 277(1), 50-55 (2007-11-08)
Haloferax mediterranei is a halophilic archaeon that can grow using nitrate as the sole nitrogen source. A ferredoxin that serves as the physiological electron donor to the nitrate and nitrite reductases in this assimilatory process has been characterized. The ferredoxin
Pascal Belin et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(18), 7426-7431 (2009-05-07)
The gene encoding the cytochrome P450 CYP121 is essential for Mycobacterium tuberculosis. However, the CYP121 catalytic activity remains unknown. Here, we show that the cyclodipeptide cyclo(l-Tyr-l-Tyr) (cYY) binds to CYP121, and is efficiently converted into a single major product in
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