E4274
Endotoxin Removal solution
Remove lipopolysaccharides (LPS) from plasmid DNA preparations
Synonym(s):
ET removal buffer, ET removal reagent, ET removal solution, endo removal buffer, endo removal reagent, endo removal solution, endotoxin contamination removal, endotoxin removal buffer, endotoxin removal reagent, plasmid purification endo, plasmid purification endo free
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.52
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Quality Level
Application
Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker′s yeast RNA.
Biochem/physiol Actions
The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.
Quantity
1 volume treats 5 volumes of DNA solution (≤1mg/ml)
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Gene therapy, 1(4), 239-246 (1994-07-01)
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells)
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