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E0502

Sigma-Aldrich

Elastin–Congo red

elastase substrate

Synonym(s):

Elastin Congo red

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About This Item

MDL number:
MFCD00131000
UNSPSC Code:
12352204
NACRES:
NA.32

form

powder

storage temp.

2-8°C

General description

Elastin−Congo red is a substrate-dye conjugate and is used in agar medium to probe elastase activity. The presence of elastase functionality leads to cleavage of the dye from the elastin substrate.

Application

Elastin−Congo red has been used as a substrate for neutrophil elastase, salivary elastase and P. aeruginosa.

Other Notes

Elastin impregnated with Congo red dye.

Substrates

Substrate for the estimation of elastase.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350,H361d

Hazard Classifications

Carc. 1B - Repr. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Activation pattern of neutrophils from blood of elderly individuals with Candida-related denture stomatitis
Gasparoto TH, et al.
European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 31(6), 1271-1277 (2012)
Salivary immunity in elderly individuals presented with Candida-related denture stomatitis
Gasparoto T, et al.
Gerodontology, 29(2), e331-e339 (2012)
AiiM lactonase strongly reduces quorum sensing controlled virulence factors in clinical strains of Pseudomonas aeruginosa isolated from burned patients
Lopez-Jacome LE, et al.
Frontiers in Microbiology, 10, 2657-2657 (2019)
Development of an agar diffusion method to measure elastase inhibition activity using Elastin-Congo red
Jung KH and Kim HJ
Journal of microbiology and biotechnology, 16(8), 1320-1324 (2006)
Martina Ramella et al.
American journal of translational research, 9(12), 5485-5495 (2018-01-10)
Progression of abdominal aortic aneurysm (AAA) is typified by chronic inflammation and extracellular matrix (ECM) degradation of the aortic wall. Vascular inflammation involves complex interactions among inflammatory cells, endothelial cells (ECs), vascular smooth muscle cells (vSMCs), and ECM. Although vascular
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Articles

Elastase

Enzyme Explorer Product Application Index for Elastase. Leukocyte elastase is a 29KDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds.

Protocols

Substrates Procedures

Our General Protease Assay Procedures and Substrates overview.

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