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biological source
human lung
growth mode
Adherent
karyotype
Modal No 53-56
morphology
Epithelial
products
alpha-1-anti-trypsin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human lung adenocarcinoma
Cell Line Description
Established from the pleural effusion of a pulmonary adenocarcinoma in a 51 year-old Japanese female. LC-2/ad cells exhibit an epithelial appearance and a tendency to form small domes. Immunocytochemically the cells are positive for CEA and cytokeratins, including cytokeratin No.18. LC-2/ad produces at least two functionally active trypsin inhibitors together with several proteinases in vitro.
Application
LC-2/ad has been used to study the ability of RXDX-105 as a therapy for cancers which are affected by RET (proto-oncogene tyrosine-protein kinase receptor) changes.
Culture Medium
RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Split confluent cultures 1:2-1:4 ie seeding at 2-5 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/ EDTA ; 5%CO2; 37°C
Other Notes
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Certificates of Analysis (COA)
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Articles
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
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