MEWO

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93082609, human skin, Fibroblast

• UNSPSC Code: 41106514

Properties

• biological source: human skin
• packaging: tube of 5 μg 93082609-DNA-5UG; pkg of vial of cells 93082609-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Melanin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human malignant melanoma

Cell Line Description

Granular fibroblasts. Derived from a human melanoma by Prof. C Grose (1978). The cells support growth of varicella-zoster virus (VZV) isolates at 36°C although viral growth is optimal at 32°C.

Application

Virus studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA (0.5% trypsin in 0.53mM EDTA in modified Puck′s saline A); 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.