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product name
WEHI-274.1, 91061231
biological source
mouse blood
growth mode
Suspension
karyotype
Not specified
morphology
Monocyte
products
Expresses the MAC-1 antigen.
receptors
Fc
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse monocyte
Cell Line Description
Derived from an Abelson virus induced tumour in a BALB/c mouse. The cells express Mac-1 but not Ia and are not phagocytic.
Culture Medium
DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 1x100,000 to 1x1000,000 cells/ml. Adherent cells can be dislodged by scraping and re-established by centrifugation and subsequent resuspension; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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