MDBK Cell Line bovine
90050801
Synonym(s):
MDBK (NBL-1) Cells, Madin Darby Bovine Kidney Cells, NBL-1 Cells
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32190102
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biological source
bovine kidney
growth mode
Adherent
karyotype
Modal no. 51; hypodiploid
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Bovine kidney
Cell Line Description
Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).
Application
MDBK cell line has been used to study the expression of Eimeria bovis-derived antigens on the host cell surface membrane in vitro. It has also been used to study the ubiquitination and degradation of signal transducer and activator of transcription 1 and 2 (STAT1 and STAT2) by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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