LIM2099 cell line human
12062002, human liver (metastasis), epithelial
Synonym(s):
LIM 2099, LIM-2099
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About This Item
UNSPSC Code:
41106514
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product name
LIM2099 cell line human, 12062002
biological source
human liver (metastasis)
growth mode
adherent
morphology
epithelial
shipped in
dry ice
storage temp.
−196°C
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Cell Line Description
Human liver metastasis, moderately differentiated sclerosing adenocarcinoma. Cell Bank Australia reference number: CBA0164.
Culture Medium
RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10μM
Subculture Routine
Optimal split ratio 1:4-1:6 (seeding density 0.8 x 104 cells/cm2). Cells incubated at 37°C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5-10 min.
Other Notes
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Certificates of Analysis (COA)
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