KARPAS 384
6120602, human blood, Large clumps growing slowly in suspension
Synonym(s):
KARPAS, 384, KARPAS-384, KARPAS384
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About This Item
UNSPSC Code:
41106514
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product name
KARPAS 384, from human blood, 06120602
biological source
human blood
growth mode
Suspension
karyotype
Complex, refer to Dyer et al., 1993
morphology
Large clumps growing slowly in suspension
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
Human T-cell non-Hodgkin lymphoma
Cell Line Description
Established from peripheral blood of a 48 year-old male with widespread subcutaneous nodules. Biopsy showed high grade Non-Hodgkin lymphoma with numerous mitoses.
DNA Profile
Not specified
Culture Medium
RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)
Subculture Routine
Doubling time approximately 60 hours. Cells grow slowly in suspension in large clumps. Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 105 cells/ml. Viability may be poor on resuscitation and may decrease further within the first 48 hours, the culture may appear non-viable at this time. Clusters of cells will eventually appear but this could take 1-2 weeks, during this time large media changes are not recommended. It may take 2-3 weeks for vigorous growth to be established. At subculture perform a cell count and maintain cultures between 3-9 x 105 cells/ml, 5% CO2, 37 °C. For cryopreservation of KARPAS 384, freeze at approximately 2 x 107 cells/ml in 90% FBS + 10% DMSO.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
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