RCC4plusVHL

3112703, human kidney, Epithelial

• Synonym(s): RCC4 VHL(+) Cells, RCC4-pVHL Cells, RCC4/VHL Cells
• UNSPSC Code: 41106514

Properties

• product name: RCC4plusVHL, 03112703
• biological source: human kidney
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL

Cell Line Description

Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL. The plasmid pcDNA3-VHL confers neomycin resistance and encodes the von Hippel-Lindau (VHL) tumour suppressor gene product pVHL. The original renal carcinoma cell line RCC4 is VHL-deficient. The RCC4 plus vector alone cell line (Sigma catalogue no. 03112702) can serve as a negative control cell line to study the effect of pVHL expression from pcDNA3-VHL. These cells are less tumourigenic than corresponding cell lines lacking pVHL function.

Application

Study of tumour suppressor gene product function - von Hippel-Lindau tumour suppressor gene. Cell responses to hypoxia.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11
D5S818: 12
D7S820: 8.2
THO1: 9.3
TPOX: 11
vWA: 16

Culture Medium

DMEM + 10% FBS + 2 mM Glutamine + G418 0.5mg/ml

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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