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91A

Sigma-Aldrich

Leukocyte α-Naphthyl Acetate (Non-Specific Esterase) Kit

Kit formulated with all liquid reagents.

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About This Item

MDL number:
MFCD08705438
UNSPSC Code:
41116123
NACRES:
NA.47

Quality Level

500

form

liquid

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure is run at 37 °C and may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.

Kit Components Only

Product No.
Description
  • TRIZMAL™ 7.6 Concentrate 50 mL
  • Hematoxylin solution gill no. 3 50 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • 916α-Naphthyl Acetate Solution 10 mLSDS
  • 915Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mLSDS
  • 917Fast Blue BB Base Solution 10 mLSDS
  • 914Sodium nitrite solution 10 mLSDS

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Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - STOT SE 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

98.1 °F

Flash Point(C)

36.7 °C

Certificates of Analysis (COA)

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S A Kuznetsov et al.
The Journal of cell biology, 153(5), 1133-1140 (2001-05-31)
We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are distinguishable from skeletal stem cells found in bone marrow (stromal stem cells, "mesenchymal
Gabrielle S Le Provost et al.
The Journal of investigative dermatology, 135(1), 279-288 (2014-07-23)
During wound healing, excessive inflammation, angiogenesis, and differentiated human dermal fibroblast (HDF ) function contribute to scarring, whereas hyperpigmentation negatively affects scar quality. Over 100 million patients heal with a scar every year. To investigate the role of the beta 2

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