79390

Phosphatase, Alkaline from calf intestinal mucosa

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

• Synonym(s): Alkaline Phosphatase from calf intestinal mucosa
• CAS Number: 9001-78-9
• Enzyme Commission number: 3.1.3.1 (BRENDA, IUBMB)
• EC Number: 232-631-4
• MDL number: MFCD00131849
• UNSPSC Code: 12352204
• eCl@ss: 42010105
• NACRES: NA.54

Properties

• biological source: bovine (calf) intestinal mucosa
• form: solution (clear, colorless)
• specific activity: ~2500 U/mg protein (~10 mg/ml)
• mol wt: M_r ~140000
• technique(s): enzyme immunoassay: suitable
• impurities: 0.1 mM ZnCl₂; 1 mM MgCl₂; 3 M NaCl; 30 mM triethanolamine
• pH: ~7.6
• storage temp.: 2-8°C

Description

Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

Reconstitution

The solution can be used without dialysis (for enzyme immuno-assay).

Other Notes

Sales restrictions may apply

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves