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Sigma-Aldrich

5(6)-Carboxy-X-rhodamine

BioReagent, suitable for fluorescence

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About This Item

Empirical Formula (Hill Notation):
C33H30N2O5
CAS Number:
198978-94-8
Molecular Weight:
534.60
UNSPSC Code:
12352116
PubChem Substance ID:
329752228
NACRES:
NA.32

product line

BioReagent

Quality Level

100

mp

≥250 °C (lit.)

fluorescence

λex 570 nm; λem ~595 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4cc5CCCN6CCCc(c4Oc7c8CCCN9CCCc(cc37)c89)c56.OC(=O)c%10ccc%11C(=O)OC%12(c%13cc%14CCCN%15CCCc(c%13Oc%16c%17CCCN%18CCCc(cc%12%16)c%17%18)c%14%15)c%11c%10

InChI

1S/2C33H30N2O5/c36-31(37)20-9-10-24-23(15-20)32(38)40-33(24)25-16-18-5-1-11-34-13-3-7-21(27(18)34)29(25)39-30-22-8-4-14-35-12-2-6-19(28(22)35)17-26(30)33;36-31(37)20-9-10-21-24(17-20)33(40-32(21)38)25-15-18-5-1-11-34-13-3-7-22(27(18)34)29(25)39-30-23-8-4-14-35-12-2-6-19(28(23)35)16-26(30)33/h2*9-10,15-17H,1-8,11-14H2,(H,36,37)

InChI key

KLNOCCPJAOCRHF-UHFFFAOYSA-N

Application

5(6)-Carboxy-X-rhodamine may be used as a fluorescent dye in nucleic acid analytical research applications.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Analysis Note

partially soluble in methanol, 0.1 M Tris pH 8.0

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH2807
BCCD6949
BCCD5623
BCCC3477
BCBX5200

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P J Oefner et al.
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Using 100 mM of triethylammonium acetate as ion-pairing reagent, phosphodiester oligonucleotides labeled fluorescently at their 5' terminus could be separated successfully on alkylated nonporous 2.3-microns poly(styrene-divinylbenzene) particles by means of high-resolution liquid chromatography. Applying excitation wavelengths of 490, 520, 550

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