16630

Atto 680-Streptavidin

BioReagent, suitable for fluorescence

• Synonym(s): Streptavidin-Atto 680
• MDL number: MFCD04040688
• UNSPSC Code: 12352108
• NACRES: NA.32

Properties

• product line: BioReagent
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• solubility: H₂O: soluble (readily soluble)
• suitability: corresponds for gel electrophoresis; suitable for fluorescence
• storage temp.: −20°C

Description

Application

Atto 680 is a fluorescent label with high molecular absorption (125.0) and quantum yield (0.30), as well as a sufficient Stoke′s shift (excitation maximum 680 nm, emission maximum 700 nm). It has limited cis-trans-isomerization, which enhances its brightness and reproducibility. Conjugation of Atto 680 to steptavidin does not significantly alter the label′s spectral data regarding excitation and emission maxima. Additionally, the molar absorbance and quantum yield remain high. Such conjugation is useful as a secondary detection reagent in histochemical applications, flow cytometry, microarrays, blot analysis, and immunoassays. Atto 680-streptavin does not show strong fluorescence until bound to biotin.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves