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08124

Sigma-Aldrich

Isoamylase from Pseudomonas sp.

ammonium sulfate suspension, ≥10,000,000 units/mg protein

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About This Item

Enzyme Commission number:
3.2.1.68 (BRENDA, IUBMB)
MDL number:
MFCD00131418
UNSPSC Code:
12352204
NACRES:
NA.54

form

ammonium sulfate suspension

Quality Level

100

specific activity

≥10,000,000 units/mg protein

storage temp.

2-8°C

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Application

Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.

Biochem/physiol Actions

Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.
Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Unit Definition

One unit causes an increase in A610 of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)

Analysis Note

≥10 MU/mg protein

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Statements

H302 + H332 - H311 - H411

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Digestion properties of corn starch modified by $\alpha$-D-glucan branching enzyme and cyclodextrin glycosyltransferase
Li Y, et al.
Food Hydrocolloids, 89, 534-541 (2019)
Lucio Gullo et al.
Pancreas, 38(4), 396-400 (2009-03-20)
To determine the type and frequency of pancreatic lesions detected by magnetic resonance cholangiopancreatography (MRCP) in subjects with asymptomatic pancreatic hyperenzymemia and to assess for a possible relationship between these lesions and the hyperenzymemia. From January 2005 to May 2008
Sebastian Streb et al.
The Plant cell, 20(12), 3448-3466 (2008-12-17)
Several studies have suggested that debranching enzymes (DBEs) are involved in the biosynthesis of amylopectin, the major constituent of starch granules. Our systematic analysis of all DBE mutants of Arabidopsis thaliana demonstrates that when any DBE activity remains, starch granules
Hiroki Takata et al.
Carbohydrate research, 344(5), 654-659 (2009-02-17)
Glycogen, highly branched (1-->4)(1-->6)-linked alpha-d-glucan, can be extracted from natural sources such as animal tissues or shellfish (natural source glycogen, NSG). Glycogen can also be synthesized in vitro from glucose-1-phosphate using the cooperative action of alpha-glucan phosphorylase (GP, EC 2.4.1.1)
Hongjie An et al.
Journal of food science, 76(1), N11-N14 (2011-05-04)
Starch debranching is fundamental for understanding the structure-function relationships of starch. In this paper, atomic force microscopy (AFM) was used to investigate potato starch by isoamylase [EC 3.2.1.68] debranching at nanometer scale. The hydrolysates were separated by gel-permeation chromatography and
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