T8907
Tryptic Soy Broth - Dehydrated Culture Media
NutriSelect® Basic, powder, suitable for microbiology
Synonym(s):
Soybean Casein digest Broth, Casein Soya Broth, CASO Broth, TSB, CASO Broth, Casein Soya Broth, Soybean Casein digest Broth, TSB, Tryptone Soya Broth
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About This Item
Recommended Products
sterility
non-sterile
Quality Level
form
powder
quality
Microbiologically tested.
packaging
poly bottle of 1 kg
poly bottle of 500 g
manufacturer/tradename
NutriSelect® Basic
application(s)
environmental
food and beverages
pathogen testing
pharmaceutical
microbiology
suitability
bacteria
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General description
Tryptic Soy Broth is a general-purpose liquid medium used in qualitative procedures for the sterility testing, enrichment, and cultivation of fastidious microorganisms. The formulation contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids and long-chained peptides. Sodium chloride maintains the osmotic equilibrium and dextrose serves as a source of carbohydrate and energy.
Application
Tryptic Soy Broth is a widely used medium for culturing aerobic gram-positive, gram-negative bacteria, fungus, routine blood cultures, and microbial biofilms. It is also been used as a growth medium to culture Staphylococcus aureus strains and as a growth medium for E.coli in the bacterial-killing assay (BKA) with bird plasma samples.
Components
Casein (pancreatic digest) 17 g/L
Soya peptone (papaic digest) 3 g/L
Sodium chloride 5 g/L
Dipotassium phosphate 2.5 g/L
Dextrose 2.5 g/L
Soya peptone (papaic digest) 3 g/L
Sodium chloride 5 g/L
Dipotassium phosphate 2.5 g/L
Dextrose 2.5 g/L
Preparation Note
Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Sisi Gao et al.
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