11746081001

Nitrite/Nitrate, Colorimetric Test

sufficient for ~64 assays, pkg of 1 tests (5 components), suitable for water monitoring

• Synonym(s): Nitrate, Nitrite, colormetric test
• UNSPSC Code: 12352200

Properties

• usage: sufficient for ~64 assays
• packaging: pkg of 1 tests (5 components)
• manufacturer/tradename: Roche
• application(s): water monitoring
• storage temp.: 2-8°C

Description

General description

Linearity
The method is linear in the range from 0.02 mg nitrite or nitrate/l sample solution to 5.00 mg nitrite or nitrate/l sample solution.

Detection limit: 0.02mg/l for nitrite and nitrate

Specificity

Under the provided conditions, nitrate reductase reacts specifically with nitrate ions.

Application

Semimicro method for the determination of nitrite and/or nitrate to investigate water, waste water, environmental samples, plant material, foodstuffs, drugs, cosmetics, and biological samples. Nitrite/Nitrate, Colorimetric Test was used in a study done to estimate the in vivo effects of myrtle oil (Myrtii oleum) on the antioxidant enzymes such as catalase and superoxide dismutase in alloxan and normoglycaemic diabetic rabbits.

Packaging

1 test combination containing 5 components.

Preparation Note

Working solution: Preparation of the solutions

• Use the contents of bottle #1 undiluted, bring to 15 to 25 °C before use.
• Place one Coenzyme-tablet in a beaker and dissolve in 3 ml of the solution from bottle 1. Remove the Coenzyme-tablet using the tweezers provided. The resulting solution is reaction mixture 2 and is sufficient for 12 nitrate determinations, bring to 15 to 25 °C before use.
• Dissolve the contents of one bottle #3 in 0.7 ml of double-dist. water to give solution 3.
• Use the contents of one bottle #4 undiluted.
• Use the contents of one bottle #5 undiluted.

Storage conditions (working solution):

• Prepare Reaction Mixture 2 immediately before use.
• Solution of Nitrate reductase is stable at 2 to 8 °C for 2 weeks.
• Solution of Color reagent I is stable at 2 to 8 °C for 3 months.
• Solution of Color reagent II is stable at 2 to 8 °C for 3 months.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• Potassium phosphate buffer, stabilized, pH 7.5
• NADPH, tablets, each containing NADPH and stabilizers
• Nitrate reductase, lyophilized, 8 U
• Color Reagent I, consisting of sulfanilamide and stabilizers
• Color Reagent II, consisting of N-(1-naphthyl)-ethylenediamine dihydrochloride and stabilizers

Safety Information

• Pictograms: GHS05,GHS08
• Signal Word: Danger
• Hazard Statements: H290 - H317 - H318 - H334
• Precautionary Statements: P261 - P280 - P284 - P304 + P340 - P305 + P351 + P338 + P310 - P342 + P311
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash