Skip to Content
Merck
All Photos(1)

Documents

10812846001

Roche

Tris hydrochloride

>99% (titration), pH 7.0-9.0, suitable for FISH

Synonym(s):

Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
Beilstein:
3675235
MDL number:
MFCD00012590
UNSPSC Code:
12352200
PubChem Substance ID:
329749168

Assay

>99% (titration)

form

solid

packaging

pkg of 500 g

manufacturer/tradename

Roche

technique(s)

FISH: suitable

pH

7.0-9.0

useful pH range

7.0-9.0

pKa (25 °C)

8.1

absorption

<0.02 at 300 nm at 100 mg/mL

storage temp.

room temp

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer.

Features and Benefits

Crystals

Quality

Purity: >99% Tris-HCl (titrimetric)
Heavy metals: <1 ppm Pb and Fe

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

MNase Digestion for Nucleosome Mapping in Neurospora.
Cigdem S
Bio-protocol null
Izabela Jęśkowiak et al.
International journal of molecular sciences, 22(20) (2021-10-24)
(1) Background: Melanoma is an aggressive neoplasm derived from melanocyte precursors with a high metastatic potential. Responses to chemotherapy and immunotherapy for melanoma remain weak, underlining the urgent need to develop new therapeutic strategies for the treatment of melanoma. (2)
Francois Charih et al.
STAR protocols, 1(3), 100135-100135 (2020-12-31)
Protein lysine methylation mediates a variety of biological processes, and their dysregulation has been established to play pivotal roles in human disease. A number of these sites constitute attractive drug targets. However, systematic identification of methylation sites is challenging and
Sen Wu et al.
Nature protocols, 3(6), 1056-1076 (2008-06-13)
We describe here a streamlined procedure for targeting vector construction, which often is a limiting factor for gene targeting (knockout) technology. This procedure combines various highly efficient recombination-based cloning methods in bacteria, consisting of three steps. First step is the
Shanker Shyam Sundhar Panchapakesan et al.
Bio-protocol, 8(7), e2799-e2799 (2018-04-05)
A native purification strategy using RNA Mango for RNA based purification of RNA-protein complexes is described. The RNA Mango aptamer is first genetically engineered into the RNA of interest. RNA Mango containing complexes obtained from cleared cellular native extracts are
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service