RMNPMAG-83K

MILLIPLEX^® Rat/Mouse Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay

The analytes available for this multiplex kit are: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: mouse, rat
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 107-135%; (Rat); standard curve range: 14-100,000 pg/mL; (Oxytocin); standard curve range: 27-20,000 pg/mL; (Neurotensin); standard curve range: 3-2,000 pg/mL; (Substance P); standard curve range: 41-30,000 pg/mL; (α-MSH); standard curve range: 69-50,000 pg/mL; (β-Endorphine)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

The MILLIPLEX^® Rat/Mouse Neuropeptide Bead Panel, containing 5 biomarkers. This kit may be used for the analysis of all or any combination of the above analytes in tissue/cell lysate/culture supernatant samples and cerebrospinal fluid (CSF). Serum or plasma samples may also be used, and will need to be extracted, however we do not recommend using the panel for serum and plasma Substance P. The kit contains enough material to analyze 38 samples in duplicate, including the standard curve and two quality controls.

An important note about this panel is that the samples may be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with the kit.

The central nervous system is a complex environment consisting of billions of neurons as well as glial support cells. These neurons use many different chemical signals to communicate information, including neurotransmitters, cannabinoids, peptides, and gases such as nitric oxide. Neuropeptides are secreted primarily from the central and peripheral nervous systems, exerting a broad spectrum of biological functions that includes the regulation of metabolism, reproduction, and immunity.

Panel Type: Neuroscience

Application

• Analytes: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P.
• Recommended Sample type: serum, plasma, CSF, and tissue culture
• Recommended Sample dilution: This assay requires 50 μL extracted serum or plasma sample per well or 50 μL neat CSF or culture media per well
• Assay Run Time: Overnight
• NOTE: this is a competitive assay.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H411
• Precautionary Statements: P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects