MABF2751

Anti-Murine Coronavirus MHV-JHM nucleocapsid Antibody, clone 1-16-1

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1-16-1, monoclonal
• mol wt: calculated mol wt 49.73 kDa; observed mol wt ~50 kDa
• purified by: using protein G
• species reactivity: SARS coronavirus
• packaging: antibody small pack of 100 μL
• technique(s): immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Unknown
• Protein ID accession no.: YP_209238
• UniProt accession no.: C0KYZ4
• shipped in: 2-8°C
• target post-translational modification: unmodified
• Gene Information: vaccinia virus ... n> N(1489757)

Description

General description

Nucleoprotein (UniProt: C0KYZ4; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene(Gene ID: 1489757) in Murine coronavirus (MHV-JHM.IA). The murine coronavirus mouse hepatitis virus (MHV), a member of the Coronaviridae family, is an enveloped virus with a single-stranded, positive-sense RNA genome. The MHV-JHM strain is shown to be highly neurovirulent and can spread in the brain in mCEACAM1a independent manner. MHV-JHM infection in susceptible weanling mice is reported to result in an encephalomyelitis when neurons, astrocytes, and oligodendrocytes are infected. MHV infection of the murine central nervous system has also been used as a model for the study of chronic demyelinating diseases. The nucleocapsid protein (N) is a basic RNA binding protein that is highly conserved among MHV strains and plays a major role in the extent of neurovirulence. It also plays structural roles by both complexing with genome RNA to form the viral capsid and interacting with the viral membrane protein (M) during virion assembly. It has three conserved regions (I, II, and III) that are separated by two hypervariable regions. It is reported to associate with microtubules, suggesting a possible role for N in trafficking and axonal transport in neurons (Ref.: Cowley, TJ., et al. (2010). J. Virol. 84(4); 1752 1763; Miura, TA., et al. (2008). J. Virol. 82(2); 755-763).

Specificity

Clone 1-16-1 is a mouse monoclonal antibody that detects nucleoprotein of Murine coronavirus (MHV-JHM.IA).

Immunogen

Robb/Lampert strain of MHV-JHM (live whole virus).

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse delayed brain tumor (DBT) cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Murine Coronavirus MHV-JHM nucleocapsid in Mouse delayed brain tumor (DBT) cell lysates.

Tested Applications

Western Blotting Analysis: A representative lot detected Murine Coronavirus MHV-JHM nucleocapsid in Western Blotting applications (Leibowitz, J.L., et al. (1987). Adv Exp Med Biol. 218; 321-31).

Immunofluorescence Analysis: A representative lot detected Murine Coronavirus MHV-JHM nucleocapsid in Immunofluorescence applications (Nanda, S.K., et al. (2001). J Virol. 75(7):3352-62).

Immunohistochemistry Applications: A representative lot detected Murine Coronavirus MHV-JHM nucleocapsid in Immunohistochemistry applications (Miura, T.A., et al. (2008). J Virol. 82(2):755-63; Navas, S., et al. (2001). J Virol. 75(5):2452-7).

Immunoprecipitation Analysis: A representative lot detected Murine Coronavirus MHV-JHM nucleocapsid in Immunoprecipitation applications (Nanda, S.K., et al. (2004). Arch Virol. 149(1):93-111).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Murine Coronavirus MHV-JHM nucleocapsid, clone 1-16-1, Cat. No. MABF2751, mouse monoclonal antibody, detects Murine Coronavirus MHV-JHM nucleocapsid and is tested in Immunofluorescence, Immunohistochemistry, Immunoprecipitation & Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable