MABC713

Anti-KEAP1, clone 7G4B10 Antibody

clone 7G4B10, from mouse

• Synonym(s): Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2, Kelch-like protein 19, KEAP1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 7G4B10, monoclonal
• species reactivity: human
• technique(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable
• UniProt accession no.: Q14145
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KEAP1(9817)

Description

General description

Kelch-like ECH-associated protein 1, also known as Cytosolic inhibitor of Nrf2 or INrf2 or Kelch-like protein 19, and encoded by the gene name KEAP1 or INRF2 or KLHL19, acts as a substrate adapter protein for the E3 ubiquitin ligase complex and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 shuttles between the cytoplasm and the nucleus and is widely expressed with high levels found in skeletal muscle.

Immunogen

Purified recombinant fragment of human KEAP1 expressed in E. Coli

Application

Anti-KEAP1, clone 7G4B10 Antibody is an antibody against KEAP1 for use in IHC, flow cytometry & ELISA.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected KEAP1 in HeLa cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected KEAP1 in bladder cancer and cerebellum tissues.

Target description

70 kDa calculated

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable