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Key Documents

MAB1668

Sigma-Aldrich

Anti-Phospholipase A2 Antibody, clone CH-7

clone CH-7, Chemicon®, from mouse

Synonym(s):

PLA2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

CH-7, monoclonal

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG2b

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Antibody reacts with cytosolic form of PLA2. Antibody will also react with beta isoform. Epitope has been localized within the first 216 aa of the N-terminal Ca++ binding domain of cPLA2

Immunogen

Recombinant cPLA2 expressed from the human cPLA2 gene.

Application

Detect Phospholipase A2 using this Anti-Phospholipase A2 Antibody, clone CH-7 validated for use in ELISA & WB.
Immunoblot: 1:500-1:1000; antibody recognizes all isoforms, the cPLA.

Alpha (85kDa) typically migrates as 100-110kDa on reducing westerns; beta migrates as 114kDa; antibody will also recognize the sPLA at approximately 14-20kDa.

ELISA

Optimal working dilutions must be determined by the end user.
Research Category
Signaling
Research Sub Category
Lipid Signaling

Physical form

Format: Purified
In 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6

Storage and Stability

Maintain at 2-8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
Haruki Nishizawa,Kanako Pryor-Koishi,Machiko Suzuki,Takema Kato,Hiroshi Kogo,Takao Sekiya et al.
Molecular Human Reproduction null
Tian Sheng Chen et al.
Nucleic acids research, 38(1), 215-224 (2009-10-24)
Intercellular exchange of protein and RNA-containing microparticles is an increasingly important mode of cell-cell communication. Here we investigate if mesenchymal stem cells (MSCs) known for secreting therapeutic paracrine factors also secrete RNA-containing microparticles. We observed that human embryonic stem cell
Clive Bate et al.
The Journal of biological chemistry, 291(1), 160-170 (2015-11-11)
The prion diseases occur following the conversion of the cellular prion protein (PrP(C)) into disease-related isoforms (PrP(Sc)). In this study, the role of the glycosylphosphatidylinositol (GPI) anchor attached to PrP(C) in prion formation was examined using a cell painting technique.

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