ABE457

Anti-c-Fos Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, rat
• species reactivity (predicted by homology): sheep (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NP_005243
• UniProt accession no.: P01100
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... FOS(2353)

Description

General description

c-Fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-Fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.

Specificity

This antibody recognizes the N-terminus of c-Fos.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human c-Fos.

Application

Anti-c-Fos Antibody is a Rabbit Polyclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB & IHC.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-Fos in rat pons and rat cerebellum tissues.

Immunofluorescence Analysis: A representative lot detected c-Fos in rat pons and rat cerebellum tissues.

Quality

Evaluated by Western Blot in PMA(TPA) treated HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos in 10 µg of PMA(TPA) treated HeLa cell lysate.

Target description

~60/56 kDa observed. This protein has multiple isoforms produced by alternative splicing ranging between 53 and 68 kDa (Jurado, J., et al. (2007). BMC Molecular Biology. 8:83-96.)

Analysis Note

Control
PMA(TPA) treated HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable