AB5020
Anti-Glycine (Low Glutaraldehyde) Antibody
diluted serum, Chemicon®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
diluted serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
shipped in
wet ice
target post-translational modification
unmodified
Related Categories
Specificity
Glycine. The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable cross-reactivity (<1:1000) against glycine in peptides or proteins. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine.
Immunogen
Glycine-glutaraldehyde-BSA
Application
Detect Glycine (Low Glutaraldehyde) using this Anti-Glycine (Low Glutaraldehyde) Antibody validated for use in IH.
Immunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.5% - 2.5% glutaraldehyde for optimum detection.
*This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative). The minimum glutaraldehyde concentration for AB5020 is 0.05%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.
Optimal working dilutions must be determined by the end user.
DILUTION: Prepare enough of the AB5020 for your days use by diluting 100X with 1% GSPBT.
*This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative). The minimum glutaraldehyde concentration for AB5020 is 0.05%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.
Optimal working dilutions must be determined by the end user.
DILUTION: Prepare enough of the AB5020 for your days use by diluting 100X with 1% GSPBT.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Packaging
2000 assays
Physical form
IgG fraction in sterile 0.1M phosphate buffer. No preservative
Storage and Stability
Maintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not freeze this stock.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Deepa M Deshpande et al.
Annals of neurology, 60(1), 32-44 (2006-06-28)
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Nature Neuroscience null
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