540221

Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

• Synonym(s): PNP
• UNSPSC Code: 12352202
• NACRES: NA.42

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• form: liquid
• specific activity: ≥25 units/mg protein
• manufacturer/tradename: Calbiochem^®
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.

Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. This reaction is reversible. The human enzyme is a target for therapeutic immune response intervention.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P_i into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.

Physical form

In 50 mM Tris-HCl, pH 7.6.

Analysis Note

Single band by SDS-PAGE

Other Notes

Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable