539576

PKA, Catalytic Subunit, Bovine Heart

• Synonym(s): cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: bovine heart
• Quality Level: 100
• Assay: ≥90% (SDS-PAGE)
• form: liquid
• specific activity: ≥20,000 units/μg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): activity assay: suitable
• suitability: suitable for molecular biology
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Research area: Cell Signaling

Native PKA catalytic subunit purified from bovine heart. Protein Kinase A (PKA) is a serine protein kinase that targets serine and threonine residues.

Biochem/physiol Actions

Protein kinases are crucial for integrating signaling networks within eukaryotic cells. It plays an essential role in regulating proliferation and fate determination by influencing Hedgehog signaling transduction. Upon binding of cAMP to the PKA regulatory subunits, the catalytic subunit of PKA dissociates from the PKA holoenzyme and proceeds to phosphorylate target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit is capable of activation without the need for cAMP and is well-suited for in vitro studies of PKA phosphorylation with purified substrates or when introduced into intact cells or cell extracts.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.

Physical form

In 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Corbin, J.D., et al. 1988. Methods Enzymol159, 74.
Rannels, S.R., et al 1983. Methods Enzymol99, 55.
Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem.66, 253.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable