407950

Ionomycin, Free Acid, Streptomyces conglobatus

Ionomycin, Free Acid, Streptomyces conglobatus, CAS 56092-81-0, is a highly specific for divalent cations and more effective than A23187 (Cat. No. 100105) as a mobile ion carrier for Ca²⁺.

• Synonym(s): Ionomycin, Free Acid, Streptomyces conglobatus
• Empirical Formula (Hill Notation): C₄₁H₇₂O₉
• CAS Number: 56092-81-0
• Molecular Weight: 709.00
• MDL number: MFCD06798385
• UNSPSC Code: 12352106

Properties

• Quality Level: 100
• Assay: ≥90% (HPLC)
• form: waxy solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• solubility: DMSO: 10 mg/mL; methanol: 10 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C41H72O9/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47)/b13-11+,34-24-/t25-,26-,27+,28-,29-,30+,31+,32-,33?,36+,37?,39-,40+,41+/m1/s1
• InChI key: PGHMRUGBZOYCAA-OJFQOPKISA-N

Description

General description

Absorption max. (280 nm, MeOH): 18,600 M^-1cm^-1.

Highly specific for divalent cations (Ca²⁺ > Mg²⁺ >> Sr²⁺ = Ba²⁺). More effective than antibiotic A23187 as a mobile ion carrier for Ca²⁺. Complexes with Ca²⁺ between pH 7 and 9.5, causing significant absorption in the UV range; however, in contrast to A23187 (Cat. No. 100105), it is not fluorescent. Useful for studies of Ca²⁺ transport across biological membranes and measurement of cytoplasmic free Ca²⁺. Induces apoptotic neuronal degeneration in embryonic cortical neurons. Also induces cell cycle arrest in the G₁ interval of mature Burkitt lymphoma cell lines.

Highly specific for divalent cations and more effective than A23187 (Cat. No. 100105) as a mobile ion carrier for Ca²⁺. Complexes with Ca²⁺ between pH 7 and 9.5. In contrast to A23187, it is not fluorescent. Useful for studies of Ca²⁺ transport across biological membranes and measurement of cytoplasmic free Ca²⁺. Induces apoptotic neuronal degeneration in embryonic cortical neurons and cell cycle arrest in the G₁ phase in mature Burkitt lymphoma cell lines.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Mobile ion carrier for Ca2+

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 year at 4°C.

Other Notes

Aagaard-Tillery, K.M., and Jelinek, D.F. 1995. J. Immunol.155, 3297.
Takei, N., and Endo, Y. 1994. Brain Res. 652, 65.
Dupuy d’Angeac, A., et al. 1993. J. Cell Physiol.154, 44.
Lee, H.C. 1993. J. Biol. Chem.268, 293.
Kuznetsov, G., et al. 1992. J. Biol. Chem. 267, 3932.
Toeplitz, B.K., et al. 1979. J. Am. Chem. Soc.101, 3344.
Liu, C.M., and Hermann, T.E. 1978. J. Biol. Chem.253, 5892.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3