208706
Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda
Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 expressed in S. frugiperda insect cells.
Synonym(s):
CaM Kinase II
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About This Item
UNSPSC Code:
12352202
Recommended Products
recombinant
expressed in insect cells (S. frugiperda)
Quality Level
Assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥250,000 units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
foreign activity
Phosphatases, proteases, none detected
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. CaM kinase II is a serine/threonine kinase that phosphorylates a wide variety of intracellular proteins. Reportedly involved in the modulation of neurite growth by cAMP. Lower levels of CaM kinase II a-subunit predispose neurons to ischemic damage.
Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. Reportedly involved in the modulation of neurite outgrowth by cAMP. Lower levels of CaM kinase II α-subunit predispose neurons to ischemic damage.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.
Physical form
In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween® 20 Detergent, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Tashima, K., et al. 1996. J. Neurochem. 66, 57.
Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
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Mirza Zizak et al.
The Journal of biological chemistry, 287(16), 13442-13456 (2012-03-01)
The epithelial brush border (BB) Na(+)/H(+) exchanger 3 (NHE3) accounts for most renal and intestinal Na(+) absorption. Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) inhibits NHE3 activity under basal conditions in intact intestine, acting in the BB, but the mechanism is unclear.
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