1.52019
Chromolith® HighResolution RP-18 endcapped
L × I.D. 100 mm x 4.6 mm HPLC columns Validation Kit (3 columns from 3 different batches)
Synonym(s):
Chromolith® HR RP-18 endcapped 100-4.6 HPLC columns Validation Kit (3 columns from 3 different batches)
About This Item
Recommended Products
material
PEEK column
Quality Level
Agency
suitable for USP L1
product line
Chromolith®
feature
endcapped
endcapped
packaging
pkg of 3 ea
extent of labeling
18% Carbon loading
18% Carbon loading
parameter
200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.
technique(s)
HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)
column L × I.D.
10 cm × 4.6 mm
surface area
250 m2/g (300 m2/g)
matrix
highly porous
monolithic silica
monolithic silica
matrix active group
C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase
pore size
1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity
operating pH range
2-7.5
2-7.5
separation technique
reversed phase
Related Categories
General description
Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for high-resolution separations of complex compound mixtures on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
- Rapid separations at very low column back-pressure
- High matrix tolerance, allowing time and cost savings with simplified workflows
- Cost-savings due to extended column lifetimes
- Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [ 1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns
Packaging
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service