M6250

2-Mercaptoethanol

≥99.0%

• Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• Beilstein: 773648
• EC Number: 200-464-6
• MDL number: MFCD00004890
• UNSPSC Code: 12352100
• PubChem Substance ID: 57654402
• NACRES: NA.22

Properties

• vapor density: 2.69 (vs air)
• Quality Level: 200
• vapor pressure: 1 mmHg ( 20 °C)
• Assay: ≥99.0%
• form: liquid
• expl. lim.: 18 %
• concentration: 14.3 M (pure liquid)
• refractive index: n20/D 1.500 (lit.)
• pH: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• density: 1.114 g/mL at 25 °C (lit.)
• storage temp.: 2-8°C
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

General description

2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.

Application

2-mercaptoethanol is widely used for retarding oxidation of biological compounds in solution.

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Target Organs: Liver,Heart
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): 165.2 °F - closed cup
• Flash Point(C): 74 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves