Y0771

Anti-phospho-YAP1 (pTyr³⁵⁷) antibody, Mouse monoclonal

clone PYP-76, purified from hybridoma cell culture

• Synonym(s): Anti-COB1, Anti-YAP, Anti-YAP-1, Anti-YAP2, Anti-YAP65, Anti-YKI
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: PYP-76, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~65 kDa
• species reactivity: human
• technique(s): indirect ELISA: suitable; indirect immunofluorescence: suitable; western blot: 0.5-1 μg/mL using total cell extract of HEK-293T cells co-transfected with wt YAP and a constitutively active mutant of c-Abl (Δ1-81)
• isotype: IgM
• UniProt accession no.: P46937
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pTyr357)
• Gene Information: human ... YAP1(10413) ; mouse ... Yap1(22601)

Description

General description

Monoclonal Anti-phospho-YAP1 (pTyr357) (mouse IgM isotype) is derived from the hybridoma PYP-76 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phosphopeptide corresponding to a fragment containing (pTyr357) of human YAP1 conjugated to KLH. Yes-associated protein 1 (YAP1) is a modular adapter protein with multiple protein interaction domains that was originally identified based on its interaction with the Src-family tyrosine kinase c-Yes. YAP1 contains a proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus.

Application

Anti-phospho-YAP1 (pTyr357) antibody, Mouse monoclonal has been used in

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• immunofluorescence

Biochem/physiol Actions

YAP1 has been shown to stabilize p73 by preventing Itch-mediated ubiquitination of p73.

YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves