SRP8055

Tim-3 (human): FC (human)

recombinant, expressed in CHO cells, ≥98% (SDS-PAGE)

• Synonym(s): HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, TIM3, TIMD3
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in CHO cells
• Assay: ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: monomer 48 kDa by calculation
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: <0.06 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: Q8TDQ0
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... HAVCR2(84868)

Description

General description

The TIM (T cell/transmembrane, immunoglobulin and mucin) family plays a critical role in regulating immune responses, including allergy, asthma, transplant tolerance, autoimmunity and the response to viral infections. The unique structure of TIM immunoglobulin variable region domains allows highly specific recognition of phosphatidylserine (PtdSer), exposed on the surface of apoptotic cells. Tim-3, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. TIM-3 is preferentially expressed on Th1 and Tc1 cells, and generates an inhibitory signal resulting in apoptosis of Th1 and Tc1 cells. TIM-3 is also expressed on some dendritic cells and can mediate phagocytosis of apoptotic cells and cross-presentation of antigen. Tim-3 functions to inhibit aggressive Th1-mediated auto- and alloimmune responses. Tim-3 pathway blockade by administration of Tim-3:Fc fusion protein accelerates diabetes in nonobese diabetic mice, causes hyperproliferation of Th1 cells and Th1 cytokine release in an experimental autoimmune encephalomyelitis (EAE) model and prevents acquisition of transplantation tolerance induced by costimulation blockade.

Physical form

Lyophilized from 0.2 μm-filtered solution in PBS.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Other Notes

The extracellular domain of human Tim-3 (aa 22-200) is fused to the N-terminus of the Fc region of human IgG1.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable