SAB5900203

Alpaca Anti-hTNFR, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• UNSPSC Code: 12352203

Properties

• biological source: alpaca
• Quality Level: 100
• recombinant: expressed in (E. coli)
• conjugate: unconjugated
• antibody form: (Affinity purified VHH antibody)
• clone: TR09, monoclonal
• Assay: >95% (SDS-PAGE)
• form: lyophilized solid
• mol wt: 14.2 kDa; ~15-18 kDa (SDS PAGE under reducing condition)
• purified by: affinity chromatography (purified via Ni-charged resin)
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): ELISA: 1:1000-1:10000; immunofluorescence: 1:100-1:300; immunohistochemistry: 1:100-1:300; western blot: 1:500-1:3000
• color: colorless
• pH: ( in PBS, pH 7.4)
• isotype: VHH
• Protein ID accession no.: NP_001056.1
• UniProt accession no.: P19438
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... TNFRSF1A(7132)

Description

General description

Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a (cluste of differentiation 120a), is an ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNFα). It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). TNFR1 can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

Specificity

Human Tumor necrosis factor receptor 1 (TNFR1) / CD120a

Immunogen

Recombinant human TNFR1 (Ile22-Thr211) protein

Application

ELISA, WB, IHC, IF

Packaging

50 µg per vial / 100 µg per vial

Physical form

Purified VHH protein in buffer containing 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA

Reconstitution

Reconstitute the lyophilized protein with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage and Stability

Stable for 1 years at 4°C from date of receipt.

Other Notes

Storage at 4°C; Do not freeze.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable