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S9008

Sigma-Aldrich

Anti-Staphylococcal Enterotoxin B antibody produced in rabbit

fractionated antiserum, lyophilized powder

Synonym(s):

Rabbit Anti-SEB

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About This Item

MDL number:
MFCD00082018
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

Staphylococcus aureus

packaging

vial of 2 mL lyophilized antiserum

technique(s)

indirect ELISA: 1:150,000-1:250,000

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Staphylococcal enterotoxin B is a toxic bacterial superantigen. The staphylococcal enterotoxins family have high sequence similarity.

Immunogen

Staphylococcal enterotoxin B from Staphylococcus aureus.

Application

Anti-Staphylococcal Enterotoxin B antibody produced in rabbit has been used:
  • to conjugate with hollow gold nanosphere and as a component of lateral flow immunoassay strip
  • in antibody sandwich immunoassay
  • for coupling with magnetic beads for detection assay

Biochem/physiol Actions

Staphylococcal enterotoxin B (SEB) is a superantigen that binds to class II MHC molecules on antigen presenting cells. It stimulates large populations of T cells resulting in acute toxic shock syndrome. It is resistant to denaturation and causes outbreaks of food poisoning that includes emetic responses, diarrhea and GI inflammation.
Staphylococcal enterotoxin B compromises the immune system by triggering inflammation and cytokine release.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2.

Reconstitution

Reconstitute with 2 ml deionized water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Application of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin B
Hwang J, et al.
Nanoscale, 8(22), 11418-11425 (2016)
A rapid and sensitive magnetic bead-based immunoassay for the detection of staphylococcal enterotoxin B for high-through put screening
Alefantis T, et al.
Molecular and Cellular Probes, 18(6), 379-382 (2004)
Bacterial toxins?Staphylococcal enterotoxin B
Fries BC and Varshney AK
Microbiology spectrum, 1(2) (2013)
Lily Zhang et al.
Toxins, 5(10), 1859-1871 (2013-10-25)
The functional activity of superantigens is based on capacity of these microbial proteins to bind to both the β-chain of the T cell receptor (TcR) and the major histocompatibility complex (MHC) class II dimer. We have previously shown that a
Staphylococcal enterotoxins
Balaban N and Rasooly A
International Journal of Food Microbiology, 61(1), 1-10 (2000)
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