P4762

Papain from papaya latex

lyophilized powder, ≥10 units/mg protein

• Synonym(s): Papainase
• CAS Number: 9001-73-4
• Enzyme Commission number: 3.4.22.2 (BRENDA, IUBMB)
• EC Number: 232-627-2
• MDL number: MFCD00131791
• UNSPSC Code: 12352204
• eCl@ss: 42030311
• NACRES: NA.54

Properties

• biological source: papaya (latex)
• Quality Level: 300
• form: lyophilized powder
• specific activity: ≥10 units/mg protein
• mol wt: 23.4 kDa
• technique(s): cell culture | mammalian: suitable; single cell analysis: suitable
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C

Description

General description

Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group.

Application

Papain from papaya latex has been used:

• to digest carotid artery smooth muscle cells
• in dissecting solutions
• to produce Fab fragments of antibodies
• for cell dissociation since it has been shown to be more effective and less damaging with certain tissues
• in the enzyme cocktail for dissociating human fetal retinal tissue
• to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation
• as a supplement in neurobasal medium to produce retinal cell suspensions

Suitable for use in preparation of single cell suspension for sequencing.

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Biochem/physiol Actions

Papain with wide specificity, cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. Papain is considered to digest protein substrates more broadly than the pancreatic proteases. Papain is commonly used in cell isolation procedures as it is highly efficient and less harmful than other proteases on certain tissues. The use of papain is known to yield more viable smooth muscle cells, retaining the cell′s sensitivity to stimulants. Papain papaya latex has antifungal activity against C. albicans and is a cysteine protease.

• The pH optimum is 6.0-7.0
• Papain hydrolyzes esters and amides

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Packaging

Package size based on protein content

Unit Definition

One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.

Physical form

Lyophilized powder containing sodium acetate

Preparation Note

2× Crystallized

Other Notes

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

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Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H315 - H319 - H334 - H335
• Precautionary Statements: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves