MAK471
Urea Assay Kit III
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
Recommended Products
Quality Level
application(s)
food and beverages
detection method
colorimetric
relevant disease(s)
diabetes; renal disease; gastrointestinal diseases; endocrinological disorders; genitourinary disorders; cardiovascular diseases; orthopedic diseases
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Urea is the major end product of protein catabolism in animals. Urea is primarily produced in the liver and secreted by the kidneys. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is useful in assessing kidney function. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases (e.g., congestive heart failure, liver diseases, and diabetes). Decreased levels often indicate acute hepatic insufficiency but may also result from over vigorous parenteral fluid therapy.
Features and Benefits
- The linear detection range of the kit is 50 to 1000 µM urea in the 96-well plate assay
- Compatible with high-throughput handling systems.
- Simple procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37 °C heater is needed.
Suitability
The kit is suitable for detection of urea in biological samples such as plasma, serum, urine, bronchoalveolar lavage (BAL), and food/beverage samples such as milk.
Principle
The Urea Assay Kit III is designed to directly measure urea in biological samples. In this assay, urease converts urea to ammonia and carbon dioxide. NADH is then converted to NAD+ in the presence of ammonia, α-ketoglutarate, and glutamate dehydrogenase. The decrease in optical density at 340 nm is directly proportional to the urea concentration in the sample.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
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