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M7528

Sigma-Aldrich

Medium 199

HEPES modification, with Earle′s salts, and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Cell culture medium, M199 Medium, TCM 199

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Medium 199, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

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General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Reconstitution

Supplement with 0.1 g/L L-glutamine.

also commonly purchased with this product

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Description
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supplement

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Description
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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Marten Michaelis et al.
BMC genomics, 18(1), 889-889 (2017-11-22)
Many genes important for reproductive performance are shared by both sexes. However, fecundity indices are primarily based on female parameters such as litter size. We examined a fertility mouse line (FL2), which has a considerably increased number of offspring and
Uğur Şen et al.
Asian-Australasian journal of animal sciences, 31(6), 827-834 (2017-12-23)
The aim of this study was to compare the effects of 36.5°C and 38.5°C incubation temperatures on the maturation of bovine oocytes and developmental competence of embryos. In experiment 1, oocytes were maturated in bicarbonate-buffered TCM-199 for 22 hours in
Marten Michaelis et al.
Andrology, 8(5), 1419-1427 (2020-04-20)
Recently we described two outbred mouse lines which have been selected for high fertility. These mouse models doubled the number of offspring per litter. Although selected for a primarily female-trait of high fertility (increased litter size), we were interested whether
Wenjia Li et al.
Cell death & disease, 11(7), 599-599 (2020-08-01)
Alpha-lipoic acid (α-LA), a well-known antioxidant, was proved to active ALDH2 in nitrate tolerance and diabetic animal model. However, the therapeutic advantage of α-LA for heart failure and related signaling pathway have not been explored. This study was designed to
Carmen Almiñana et al.
Reproduction (Cambridge, England), 154(3), 153-168 (2017-06-21)
Successful pregnancy requires an appropriate communication between the mother and the embryo. Recently, exosomes and microvesicles, both membrane-bound extracellular vesicles (EVs) present in the oviduct fluid have been proposed as key modulators of this unique cross-talk. However, little is known
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Articles

Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

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Medium 199 Formulation

Medium 199 Formulation

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