I1020

Interleukin-4 from mouse

IL-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): mIL-4, Interleukin-4 human
• MDL number: MFCD00166698
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.1-2.0 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: ~13.5 kDa
• packaging: pkg of 5 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg
• UniProt accession no.: P07750
• storage temp.: −20°C
• Gene Information: mouse ... Il4(16189)

Description

Application

Interleukin-4 from mouse has been used:

• as a component of cell culture media for class switch recombination assay
• to study its therapeutic benefits for mucosal healing
• as a pioglitazone or new peroxisome proliferator-activated receptor agonist (GQ-11)

Biochem/physiol Actions

Interleukin-4 (IL-4) is a lymphokine with profound effects on the growth and differentiation of immunologically competent cells. IL-4 is also known as B cell stimulatory factor-1 (BSF-1), T cell growth factor-2 (TCGF-2) and mast-cell growth factor-2 (MCGF-2). Inhibits VEGF-induced and FGF-Basic-induced angiogenesis. IL-4 is a complex glycoprotein released by a subset of activated T cells. Treatment of IL-4 with specific glycosidases yields an active polypeptide. Human and mouse IL-4 share 50% amino acid sequence homology, but their biological actions are species-specific.

Physical form

Lyophilized, 0.2 μm filtered, with no additives.

Analysis Note

The proliferative activity is tested in culture using mouse HT-2 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves