H4281
EX-CELL® Hybridoma Medium
without antibiotics, antimycotics, L-glutamine, and phenol red, with sodium bicarbonate, HEPES, essential and non-essential amino acids, liquid, sterile-filtered, suitable for hybridoma
Pharma Manufacturing
Synonym(s):
Hybridoma Medium
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About This Item
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description
endotoxin tested
for research or for further manufacturing use
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | hybridoma: suitable
components
HEPES: yes
NaHCO3: yes
L-glutamine: no
phenol red: no
glucose: 2.9 g/L
shipped in
ambient
storage temp.
2-8°C
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Application
Serum-free, low-protein medium formulated to support the growth of hybridomas used in the manufacture of monoclonal antibodies.
Features and Benefits
Designed to support the rapid growth of cells to high densities and the maintenance of viable cells for extended culture periods. This formulation reduces raw material cost and the low protein level reduces cost associated with downstream processing. Purified components in the product reduce the variability sometimes encountered with biological materials leading to improved consistency of the medium performance. Cells can be successfully transferred from serum-supplemented cultures to this medium with little or no adaptation.
Other Notes
This proprietary formulation includes inorganic salts, essential and non-essential amino acids, vitamins, sodium bicarbonate, HEPES, trace elements, fatty acids, and other organics. It contains low concentrations of recombinant human insulin, bovine serum albumin and human transferrin (source tested negative for HIV antibody and HbsAG). The medium does not contain L-glutamine, antibiotics, antimycotics, and phenol red.
Caution
Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time. When stored at 2-8°C in the dark, medium that has not been supplemented is stable until the expiration date on the label.
Reconstitution
Prior to use, medium should be warmed to 37°C and supplemented with 50 ml/L of 200 mM L-glutamine.
Legal Information
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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