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GE28-9187-80

MBPTrap HP

Cytiva 28-9187-80, pack of 5 × 5 mL

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

ligand

dextrin

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 28-9187-80

storage condition

(20% Ehtanol)

parameter

<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-13

working range

>7

capacity

~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)

storage temp.

2-8°C

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General description

MBPTrap HP columns prepacked with Dextrin Sepharose High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP).

Features and Benefits

  • HiTrap® columns prepacked with Dextrin Sepharose High Performance medium.
  • Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
  • Physiological conditions and mild elution preserve the activity of the target protein.
  • Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
MBPTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

FlameFlame

Signal Word

WarningDanger

Hazard Statements

Hazard Classifications

Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.

This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Protocols

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service