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product name
CLC, 95070628
biological source
fish blood
growth mode
Adherent
karyotype
2n = 104
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Carp leukocyte
Cell Line Description
The cell line CLC (Carp Leukocyte Culture) was established from peripheral blood mononuclear cells of a normal 2 year old female common carp, Cyprinus carpio. They possess phagocytic activity and are reported to be stimulated by TNF, gamma-interferon and LPS therefore resembling characteristic features of monocytes / macrophages. CLC replicate fish viruses e.g. IPNV, PFR, SVCV and VHSV.
Application
Immunological research, fish virology, fish cell physiology.
Culture Medium
EMEM (EBSS) or TC-199 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Spilt confluent cultures 1:3 using 0.25% trypsin or trypsin/EDTA; 25-28°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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