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Product Name
SW 1417, 90102543
biological source
human colon
growth mode
Adherent
karyotype
2n = 46
morphology
Epithelial
products
Carcinoembryonic antigen (CEA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human colon adenocarcinoma
Cell Line Description
Derived from a grade III adenocarcinoma of the colon in a 53 year old female. CEA is produced and electron microscopy reveals the presence of brush borders around colonies in vitro.
Application
Tumourigenicity studies
Culture Medium
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; No CO2; 37°C. Centrifugation and resuspension are recommended to disperse clumps.
Other Notes
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