SCC-4
89062002, human tongue, squamous cell carcinoma. Epithelial morphology.
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About This Item
UNSPSC Code:
41106514
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product name
SCC-4, 89062002
biological source
human mouth
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Epidermal keratins; 40 kD Keratin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human tongue squamous carcinoma
Cell Line Description
Derived from a human squamous cell carcinoma (SCC) of the tongue from a 55-year-old male. SCC-4 cells have been reported to form colonies in semi-solid medium, and are not induced to differentiate by anchorage deprivation. Growth is enhanced by use of a feeder layer of 3T3 swiss cells.
Culture Medium
DMEM:HAMS F12 (1:1) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 0.4 μg/ml hydrocortisone
Subculture Routine
Split confluent cultures 1:3 to 1:4, i.e., seeding at 3-5 x 10,000 cells/cm2 using trypsin/EDTA; 5% CO2; 37°C
Other Notes
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Certificates of Analysis (COA)
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