LLC-WRC 256
87061101, rat unknown/unspecified, Epithelial
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product name
LLC-WRC 256, 87061101, from rat unknown/unspecified
biological source
rat unknown/unspecified
growth mode
Adherent
karyotype
Modal no. 41 (2n=42)
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Walker carcinoma epithelial
Cell Line Description
Walker rat tumour cell line, which supports the growth of herpes simplex, pseudorabies and vaccinia viruses. The cells are highly tumourigenic in suckling rats.
Culture Medium
Medium 199 + 2mM Glutamine + 5% Horse Serum (HS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C
Other Notes
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Certificates of Analysis (COA)
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