94A1

Application: Fusion partner, 85062503

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Bovine Heterohybridoma
• ImmunoDon: Jersey calf lymph node
• Myeloma: 94A1 (mouse x bovine heteromyeloma)
• Reactivity: Application: Fusion partner
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse 53B3 x Bovine Jersey calf lymph node cells

Cell Line Description

The antibody is directed against sheep red blood cells. It does not react with bovine red cells. This haemocytic antibody is probably directed against the Forssman hapten. This HAT sensitive line can be used as a fusion partner for the production of heterohybridomas.

Immunogen

Sheep red blood cells

Application

Fusion partner

The isotype of the monoclonal antibody produced by this cell line is bovine IgG1.

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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