MDCK Cell Line canine
84121903, canine kidney, Epithelial
Synonym(s):
MDCK (NBL-2) Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32190102
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product name
MDCK Cell Line canine, 84121903
biological source
canine kidney
form
cell suspension
growth mode
Adherent
karyotype
2n = 78
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Canine Cocker Spaniel kidney
Cell Line Description
From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.
Application
Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.
Other Notes
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Certificates of Analysis (COA)
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