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C2999

Sigma-Aldrich

CryoStor® cell cryopreservation media

CS5

Synonym(s):

cell freezing medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

100

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

shipped in

ambient

storage temp.

2-8°C

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General description

The CryoStor CS2, CS5, and CS10 family of preservation solutions represents the next generation of cryopreservation media. Designed to prepare and preserve cells in ultra low temperature environments (-80 to -196 °C), CryoStor media provide a safe, protective environment for cells and tissues during the freezing, storage, and thawing process. Through modulating the cellular biochemical response to the cryopreservation process, these media provide enhanced cell viability and functionality, while eliminating the need to include serum, proteins, or high levels of cytotoxic agents.

CryoStor CS2, CS5, and CS10 are a series of cell specific, optimized preservation media, uniquely formulated to address the molecular biological aspects of cells during the cryopreservation process; thereby, directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.

These media are recommended for the preservation of stem cells, hepatocytes, tissue samples, and other extremely sensitive cell types.

Application

CryoStor, a series of cell-specific, optimized preservation media, is uniquely formulated to address the molecularbiological aspects of cells during the cryopreservation process thereby directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.
Cryostor CS5 is formulated to contain 5% dimethyl sulfoxide (DMSO). Recommended for cryopreservation of most cell types.

Cryostor® Cryopreservation Video Protocol

Other Notes

Formulation contains 5% DMSO.

Legal Information

CryoStor is a registered trademark of BioLife Solutions, Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hui Li et al.
Journal of virology (2021-03-05)
Previously, we showed that substitution of HIV-1 Env residue 375-Ser by bulky aromatic residues enhances binding to rhesus CD4 and enables primary HIV-1 Envs to support efficient replication as simian-human immunodeficiency virus (SHIV) chimeras in rhesus macaques (RMs). Here, we
J Stylianou et al.
Cytotherapy, 8(1), 57-61 (2006-04-22)
Hematopoietic stem cells (HSC) have traditionally been frozen using the cryoprotectant DMSO in dextran-40, saline or albumin. However, the process of freezing and thawing results in loss of HSC numbers and/or function. This study investigated the use of CryoStor for
Dominic M Clarke et al.
Cytotherapy, 11(4), 472-479 (2009-06-06)
Peripheral blood stem cells (PBSC) have become the preferred stem cell source for autologous hematopoietic transplantation. A critical aspect of this treatment modality is cryopreservation of the stem cell products, which permits temporal separation of the PBSC mobilization/collection phase from

Protocols

CryoStor® Cryopreservation Protocol

Cryopreservation efficacy which includes post-thaw recovery, viability, and functionality is of importance to both research and clinical applications. The cumulative stresses that result from the cryopreservation process and suboptimal freeze media result in cell death from necrosis and apoptosis.

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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