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Key Documents

A3429

Sigma-Aldrich

Differentiation Solution

for the differentiation of regressive hematoxylin stains

Synonym(s):

Acid Alcohol

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About This Item

MDL number:
MFCD00243254
UNSPSC Code:
12171500
NACRES:
NA.47

form

solution

shelf life

40 mo.

color

colorless

application(s)

hematology
histology

storage temp.

room temp

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General description

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

Application

Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
Differentiation solution is employed mainly for the differentiation of regressive hematoxylin stains. It has been used in the following studies:

  • the prediction of pulmonary metastasis progression in osteosarcoma patients
  • the development of multispectral imaging to detect melanin

Features and Benefits

  • Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
  • Results are sharper and more rapid compared to other differentiation methods.
  • Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.

Principle

In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.

Signal Word

Danger

Hazard Statements

H225,H290,H319,H371

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

71.6 °F

Flash Point(C)

22 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
SLCC1858
SLBV9979
SLBS9606
SLBK6546V
SLBD9571V

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