A2934
Bovine Serum Albumin
lyophilized powder, essentially globulin free, low endotoxin, ≥98% (agarose gel electrophoresis)
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
biological source
bovine
Quality Level
Assay
≥98% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable
impurities
essentially globulin free
≤1 ng/mg endotoxin
pH
5.2
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT Virus, none detected
VSV Virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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General description
Bovine Serum Albumin (BSA) is a globular non-glycosylated protein that encompasses structurally distinct domains (I, II, and III) with two sub-domains, A and B within each domain. Structurally, BSA is an α-helical protein with 17 cysteine residues.
Application
Bovine Serum Albumin has been used:
- as a component of the minimal liquid medium to culture Porphyromonas gingivalis strains
- as a standard protein to quantify microsomes homogenate using Bradford method
- as a blocking agent in enzyme-linked immunosorbent assay (ELISA) of fecal lactoferrin
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is widely used in drugs and antibody delivery. It also finds applications in nanoparticle preparations and is useful in targeting tumor tissues. BSA is non-immunogenic and non-toxic. It also shows binding with metal ions.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
- Low endotoxin
- Essentially globulin-free
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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