Skip to Content
Merck
All Photos(1)

Key Documents

M0678

Millipore

Middlebrook OADC Growth Supplement

Enrichment supplement recommended for isolation and cultivation of Mycobacteria, suitable for microbiology

Synonym(s):

Middlebrook Oleic Albumin Dextrose Catalase Growth Supplement, Oleic Albumin Dextrose Catalase

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41171614
NACRES:
NA.85

Quality Level

sterility

sterile (Filtered and Aseptic Handled)

form

liquid

shelf life

limited shelf life, expiry date on the label

packaging

vial of 50 mL

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
veterinary

microbiology

storage temp.

2-8°C

suitability

mycobacteria

General description

Middlebrook OADC Growth Supplement is used with the Middlebrook Agar, that allows the isolation and cultivation of a wide variety of Mycobacteria. Middlebrook OADC Growth Supplement contains oleic acid, bovine albumin, sodium chloride, dextrose, and catalase. Oleic acid and other long chain fatty acids are essential for the metabolism of Mycobacteria. Some free fatty acids are toxic to Mycobacteria, but albumin binds to those fatty acids and prevents toxic action on Mycobacteria. Dextrose is the fermentable carbohydrate and an energy source whereas catalase destroys toxic peroxides that may be present in the medium.

Application

Middlebrook OADC growth supplement has been used as a supplement in 7H9 liquid medium to culture mycobacteria from clinical samples, environmental, food and beverages.

Components

Ingredients: (per vial)
Bovine Albumin Fraction V 2.50 g
Dextrose 1.00 g
Catalase 0.002 g
Oleic acid 0.025 g
Sodium chloride 0.425 g
Distilled water 50 mL

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Recombinant BCG Vaccines Reduce Pneumovirus-Caused Airway Pathology by Inducing Protective Humoral Immunity
Soto JA, et al.
Frontiers in Immunology, 9 (2018)
Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy
Antibiotic resistance protocols, 41-50 (2018)
Recombinant BCG Vaccines Reduce Pneumovirus-Caused Airway Pathology by Inducing Protective Humoral Immunity
Soto JA, et al.
Frontiers in immunology, 9 (2018)
Shuxin Liang et al.
Autophagy, 16(8), 1453-1467 (2019-11-13)
BAG2 (BCL2 associated athanogene 2) is associated with cell fate determination in response to various pathological conditions. However, the effects of BAG2 on M. tuberculosis-induced endoplasmic reticulum (ER) stress remain elusive. Herein, we report that M. tuberculosis infection of macrophages
Susanta Pahari et al.
Autophagy, 16(6), 1021-1043 (2019-08-30)
Host-directed therapies are gaining considerable impetus because of the emergence of drug-resistant strains of pathogens due to antibiotic therapy. Therefore, there is an urgent need to exploit alternative and novel strategies directed at host molecules to successfully restrict infections. The

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service